|Release date:||October 10, 2014|
|Running time:||22 minutes|
"Halloween Night at the Museum" originally aired on 10 October 2014, and is the second Ultimate Spider-Man Halloween episode.
- This episode is a crossover with one of Disney Channel's live-action sitcoms - Jessie.
|Drake Bell||Peter Parker / Spider-Man|
|Cameron Boyce||Luke Ross|
|Karan Brar||Ravi Ross|
|Grey Griffin||Morgan Le Fay|
|Skai Jackson||Zuri Ross|
|Peyton List||Emma Ross|
|Debby Ryan||Jessie Prescott|
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